Possible physiological functions of penicillin-binding proteins in Staphylococcus aureus
نویسندگان
چکیده
منابع مشابه
Kinetics of penicillin binding to penicillin-binding proteins of Staphylococcus aureus.
Reduced affinity of penicillin-binding proteins (PBPs) for binding penicillin has been proposed as a mechanism of beta-lactam antibiotic resistance in staphylococci. Penicillin binding by PBPs of three penicillin-susceptible and two penicillin-resistant strains of Staphylococcus aureus was studied in kinetic assays to determine rate constants, drug concentrations at which PBPs were bound and th...
متن کاملHow allosteric control of Staphylococcus aureus penicillin binding protein 2a enables methicillin resistance and physiological function.
The expression of penicillin binding protein 2a (PBP2a) is the basis for the broad clinical resistance to the β-lactam antibiotics by methicillin-resistant Staphylococcus aureus (MRSA). The high-molecular mass penicillin binding proteins of bacteria catalyze in separate domains the transglycosylase and transpeptidase activities required for the biosynthesis of the peptidoglycan polymer that com...
متن کاملDirect quantitation of the numbers of individual penicillin-binding proteins per cell in Staphylococcus aureus.
The penicillin-binding proteins (PBPs) are a set of enzymes that participate in bacterial peptidoglycan assembly. The absolute numbers of each PBP were determined by direct measurement and have been reported for two Staphylococcus aureus strains, RN4220 (methicillin-sensitive S. aureus) and RN450M (methicillin-resistant S. aureus). From the specific activity of the labeled penicillin and the ab...
متن کاملEffects of neutrophils on cefazolin activity and penicillin-binding proteins in Staphylococcus aureus abscesses.
Bacteria survive within abscesses despite antimicrobial therapy, usually necessitating drainage. Our previous work showed that bacterial killing is diminished within the neutrophils of animals with abscesses. To further assess the role of neutrophils in Staphylococcus aureus survival and the poor activities of beta-lactams in abscesses, tissue cage abscess-bearing rats were given polymorphonucl...
متن کاملAltered penicillin-binding proteins in methicillin-resistant strains of Staphylococcus aureus.
The penicillin-binding proteins (PBPs) of a methicillin-resistant (MR) and a methicillin-susceptible (MS) Staphylococcus aureus were compared by various approaches involving the use of high-specific-activity [3H]penicillin as a reagent. The MR and MS strains were found to contain PBPs of the same number and electrophoretic mobilities. However, saturation of PBPs 1, 2, and 3 by methicillin in th...
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 1986
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.29.2.333